KoiZyme is a biological formula of naturally occurring bacteria, enzymes and micro nutrients. When used as directed, KoiZyme will reduce the bacterial count of Aeromonas, Pseudomonas and other pathnogenetic bacteria to such low levels that they will not be a threat to the health of your koi. 1 Pint treats 24,000 to 48,000 gallons Five initial treatments are required and then one a week maintenance treatments will keep your pond healthy KoiZyme should be used when pond water temps reach 52 degrees and above KoiZyme will NOT harm the biofilter and can be used with any percentage of salt Shelf life of KoiZyme: 4 to 6 months at room temps and 18 months if kept refrigerated. koizyme1pint Most Orders Shipped Out Same Day! Premier Ponds LLC pondinfo@premierponds.com U.S.A. Copyright © 1999 - 2007 Premier Ponds LLC, North Salem, New York. All rights reserved. Thermo-Pond Heater 3.0, Aquascape Designs Pond Kits, ScareCrow Sprinkler, Nursery Pro Micro Pond Kits, AquaClearer, Microbe-Lift, Great Blue Heron Decoy, Pond Liners, Thermo Pond Heater & Deicer, Premium Koi Food, Select Koi, Butterfly Koi, Pond Aeration Pumps, Flexible PVC Piping, BioFalls, Skimmer, Aquascape Pumps, Cat Stop!, Animal Control Products, Pond Netting.